• Dr Hee-Jeon Hong

    BEng, MEng, DPhill

    Senior Lecturer in Microbiology

    Department of Biological and Medical Sciences

    Faculty of Health and Life Sciences

    Hee-Jeon 2

    Phone number: 01865 483746

    Email: hee-jeon.hong@brookes.ac.uk

    Location: Office: Helena Kennedy F126a, Lab: Tonge T123

    Appointments

    2016 - present   Senior Lecturer, Department of Biological and Medical Sciences, Oxford Brookes University, UK
    2006 - 2015      Research Group Head, Royal Society University Fellow, Department of Biochemistry, University of Cambridge, UK
    2002 - 2006      Postdoctoral research fellow, John Innes Centre, UK

     

    Qualifications

    1999 - 2002      Doctor of Philosophy, Biological Science, John Innes Centre/University of East Anglia, UK
    1996 - 1998      PhD course completion, Biochemical Engineering, Seoul National University, South Korea
    1994 - 1996      Master of Engineering, Biotechnology, Inha University, South Korea
    1990 - 1994      Bachelor of Engineering, Biotechnology, Inha University, South Korea

    Modules taught

    MSc Infection, Prevention and Control (Course Leader)

    P44617 Infection, Prevention and Control (Module Leader)

     

    Other Modules taught/involved

    Undergraduate modules 

    U14514 Scientific Skills  
    U14515 Professional and Experimental Skills
    U14525 Biochemistry of Cell Function  
    U14533 Microbiology  
    U14582 Infection, Immunity and Immnunology    
    U14699 BMS Undergraduate Project

    Postgraduate modules

    P10114 Clinical Genetics and Diagnostics  
    P10188 Independent Study                                    
    P44615 Communicable Disease
    P44621 Disseration for Infection Prevention and Control  
    P44622 Applied Epidemiology for Infection Prevention and Control

    Supervision

    Supervising a PhD research student in microbiology

     

    * Please feel free to contact me if you wish to discuss about PhD/MSc project in my group.

    The development of resistance to existing antibiotics, coupled with a sustained decline in the success rate of the discovery of new ones, is currently leading to a point in the future where many infections could essentially be untreatable by the compounds that are available. A fundamental understanding of bacterial antibiotic tolerance and resistance mechanisms will be an important part of any future strategy aimed at solving the growing problems with treating microbial infections. We are using a harmless, soil derived Gram-positive bacteria, Actinomycetes, as a model system to investigate the mechanisms involved in sensing, responding and adapting to antibiotic attack. Actinomycetes produce about 70% of known antibiotics and are the ultimate source of most antibiotic resistance genes. Consequently they possess many genes involved in sensing and responding to extracellular antibiotics, and are an ideal system to use for furthering the understanding of the processes involved. Primarily, we will focus on the mechanisms underlying changes in the bacterial cell wall caused by exposure to antibiotics. The bacterial cell wall is crucial for normal cell growth, and many antibiotics that target the cell wall find use in the treatment of infectious diseases e.g. penicillin and vancomycin. Understanding the mechanisms by which bacterial cells respond to cell envelope damage is important for the future development of effective antibiotics, and for prolonging the therapeutic use of existing drugs. How damage caused externally by antibiotics inhibiting cell wall biosynthesis is communicated to the bacterial chromosome, and how the subsequent reprogramming of gene expression acts to counteract the damage is the major focus of my research. Our research involves using functional genomics approaches including transcriptome and proteome profiling to study and characterise the response when growing cultures of bacterial cells are challenged with antibiotics. Ultimately, understanding the dynamic link between transcriptional and translational processes will extend our knowledge of the functions and systems that are important for bacterial cell wall homeostasis, and open ways by which these can be exploited in future antibiotic therapies. We also aim to progress this research into pathogens responsible for hospital acquired infections. This work has direct implications for public health, contributing to efforts to understand the molecular basis of defensive responses and resistance to antibiotics in bacteria.

    https://cms-staging.brookes.ac.uk/BMS/Research/Groups/Biomedical-science/Microbiology/Bacterial-Functional-Genomics/

    Research group membership

    Sam Connelly (PhD student)

    Research grants and awards

    2011 - 2015   Royal Society University Research Fellowship (renewal)
    2008 - 2010   Medical Research Council Collaboration Grant (co-applicant)
    2007 - 2011   Medical Research Council New Investigator Research Grant
    2006 - 2011   Royal Society University Research Fellowship
    2003 - 2006   Biotechnology and Biological Sciences Research Council Research Grant (co-wrote)
    1998 - 1999   Korea Research Foundation Research Grant for young PhD scientist

    Journal articles

    • Hong H, 'Construction of a Bioassay System to Identify Extracellular Agents Targeting Bacterial Cell Envelope'
      Methods in Molecular Biology 1440 (2016) pp.125-137
      ISSN: 1064-3745 eISSN: 1064-3745
      Abstract Website
    • Hong HJ, Hesketh A, 'Microarray Analysis to Monitor Bacterial Cell Wall Homeostasis.'
      Methods in molecular biology (Clifton, N.J.) 1440 (2016) pp.31-46
      ISSN: 1064-3745 eISSN: 1064-3745
      Abstract Website
    • Cha JY, Han S, Hong HJ, Cho H, Kim D, Kwon Y, Kwon SK, Crusemann M, Lee YB, Kim JF, Giaever G, Nislow C, Moore BS, Thomashow LS, Weller DM, Kwak YS, 'Microbial and biochemical basis of a Fusarium wilt-suppressive soil'
      ISME Journal 10 (2016) pp.119-129
      ISSN: 1751-7362 eISSN: 1751-7362
      Abstract Website
    • Zarkan A, Macklyne HR, Truman AW, Hesketh AR, Hong HJ, 'The frontline antibiotic vancomycin induces a zinc starvation response in bacteria by binding to Zn(II)'
      SCIENTIFIC REPORTS 6 (2016)
      ISSN: 2045-2322 eISSN: 2045-2322
      Abstract Website
    • Novotna GB, Kwun MJ, Hong HJ, 'In Vivo Characterization of the Activation and Interaction of the VanR-VanS Two-Component Regulatory System Controlling Glycopeptide Antibiotic Resistance in Two Related Streptomyces Species'
      Antimicrobial Agents and Chemotherapy 60 (3) (2016) pp.1627-1637
      ISSN: 0066-4804 eISSN: 0066-4804
      Abstract Website
    • Hesketh A, Deery MJ, Hong HJ, 'High-Resolution Mass Spectrometry Based Proteomic Analysis of the Response to Vancomycin-Induced Cell Wall Stress in Streptomyces coelicolor A3(2)'
      JOURNAL OF PROTEOME RESEARCH 14 (2015) pp.2915-2928
      ISSN: 1535-3893 eISSN: 1535-3893
      Abstract Website
    • Kwun MJ, Hong HJ, 'The Activity of Glycopeptide Antibiotics against Resistant Bacteria Correlates with Their Ability To Induce the Resistance System'
      ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 58 (10) (2014) pp.6306-6310
      ISSN: 0066-4804 eISSN: 0066-4804
      Abstract Website
    • Truman AW, Kwun MJ, Cheng J, Yang SH, Suh JW, Hong HJ, 'Antibiotic Resistance Mechanisms Inform Discovery: Identification and Characterization of a Novel Amycolatopsis Strain Producing Ristocetin'
      ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 58 (10) (2014) pp.5687-5695
      ISSN: 0066-4804 eISSN: 0066-4804
      Abstract Website
    • Kwun MJ, Hong HJ, 'Genome Sequence of Streptomyces toyocaensis NRRL 15009, Producer of the Glycopeptide Antibiotic A47934.'
      Genome Announcements 2 (4) (2014)
      ISSN: 2169-8287 eISSN: 2169-8287
      Abstract Website
    • Kwun MJ, Hong HJ, 'Draft Genome Sequence of Amycolatopsis lurida NRRL 2430, Producer of the Glycopeptide Family Antibiotic Ristocetin.'
      Genome announcements 2 (5) (2014)
      ISSN: 2169-8287 eISSN: 2169-8287
      Abstract Website
    • Kwun MJ, Novotna G, Hesketh AR, Hill L, Hong HJ, 'In Vivo Studies Suggest that Induction of VanS-Dependent Vancomycin Resistance Requires Binding of the Drug to D-Ala-D-Ala Termini in the Peptidoglycan Cell Wall'
      ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 57 (2013) pp.4470-4480
      ISSN: 0066-4804 eISSN: 0066-4804
      Abstract Website
    • Duong A, Capstick DS, Di Berardo C, Findlay KC, Hesketh A, Hong HJ, Elliot MA, 'Aerial development in Streptomyces coelicolor requires sortase activity'
      MOLECULAR MICROBIOLOGY 83 (5) (2012) pp.992-1005
      ISSN: 0950-382X eISSN: 0950-382X
      Abstract Website
    • Novotna G, Hill C, Vincent K, Liu C, Hong HJ, 'A Novel Membrane Protein, VanJ, Conferring Resistance to Teicoplanin'
      ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 56 (4) (2012) pp.1784-1796
      ISSN: 0066-4804 eISSN: 0066-4804
      Abstract Website
    • Hesketh A, Hill C, Mokhtar J, Novotna G, Tran N, Bibb M, Hong HJ, 'Genome-wide dynamics of a bacterial response to antibiotics that target the cell envelope'
      BMC GENOMICS 12 (226) (2011)
      ISSN: 1471-2164 eISSN: 1471-2164
      Abstract Website
    • Koteva K, Hong HJ, Wang XD, Nazi I, Hughes D, Naldrett MJ, Buttner MJ, Wright GD, 'A vancomycin photoprobe identifies the histidine kinase VanSsc as a vancomycin receptor'
      Nature Chemical Biology 6 (2010) pp.327-329
      ISSN: 1552-4450 eISSN: 1552-4450
      Abstract Website
    • Kallifidas D, Pascoe B, Owen GA, Strain-Damerell CM, Hong HJ, Paget MSB, 'The Zinc-Responsive Regulator Zur Controls Expression of the Coelibactin Gene Cluster in Streptomyces coelicolor'
      JOURNAL OF BACTERIOLOGY 192 (2) (2010) pp.608-611
      ISSN: 0021-9193 eISSN: 0021-9193
      Abstract Website
    • Hong HJ, 'Studying gene induction of glycopeptide resistance using gene swapping.'
      Methods in molecular biology (Clifton, N.J.) 642 (2010) pp.45-62
      ISSN: 1064-3745 eISSN: 1064-3745
      Abstract Website
    • Hong HJ, Paget MSB, Buttner MJ, 'A signal transduction system in Streptomyces coelicolor that activates expression of a putative cell wall glycan operon in response to vancomycin and other cell wall-specific antibiotics'
      MOLECULAR MICROBIOLOGY 69 (4) (2008) pp.1069-1069
      ISSN: 0950-382X eISSN: 0950-382X
      Abstract Website
    • Hutchings MI, Hong HJ, Buttner MJ, 'The vancomycin resistance VanRS two-component signal transduction system of Streptomyces coelicolor'
      MOLECULAR MICROBIOLOGY 59 (3) (2006) pp.923-935
      ISSN: 0950-382X eISSN: 0950-382X
      Abstract Website
    • Hutchings MI, Hong HJ, Leibovitz E, Sutcliffe IC, Buttner MJ, 'The sigma(E) cell envelope stress response of Streptomyces coelicolor is influenced by a novel lipoprotein, CseA'
      JOURNAL OF BACTERIOLOGY 188 (2006) pp.7222-7229
      ISSN: 0021-9193 eISSN: 0021-9193
      Abstract Website
    • Hong HJ, Hutchings MI, Hill LM, Buttner MJ, 'The role of the novel Fem protein VanK in vancomycin resistance in Streptomyces coelicolor'
      JOURNAL OF BIOLOGICAL CHEMISTRY 280 (13) (2005) pp.13055-13061
      ISSN: 0021-9258 eISSN: 0021-9258
      Abstract Website
    • Hong HJ, Hutchings MI, Neu JM, Wright GD, Paget MSB, Buttner MJ, 'Characterization of an inducible vancomycin resistance system in Streptomyces coelicolor reveals a novel gene (vanK) required for drug resistance'
      MOLECULAR MICROBIOLOGY 52 (2004) pp.1107-1121
      ISSN: 0950-382X eISSN: 0950-382X
      Abstract Website
    • Hong HJ, Paget MSB, Buttner MJ, 'A signal transduction system in Streptomyces coelicolor that activates the expression of a putative cell wall glycan operon in response to vancomycin and other cell wall-specific antibiotics'
      MOLECULAR MICROBIOLOGY 44 (2002) pp.1199-1211
      ISSN: 0950-382X eISSN: 0950-382X
      Abstract
    • Kim ES, Hong HJ, Choi CY, Cohen SN, 'Modulation of actinorhodin biosynthesis in Streptomyces lividans by glucose repression of afsR2 gene transcription.'
      Journal of bacteriology 183 (7) (2001) pp.2198-203
      ISSN: 0021-9193 eISSN: 0021-9193
      Abstract Website
    • Hong HJ, Lee JE, Ahn JE, Kim DI, 'Enhanced production of digoxin by digitoxin biotransformation using in situ adsorption in Digitalis lanata cell cultures'
      JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 8 (1998) pp.478-483
      ISSN: 1017-7825 eISSN: 1017-7825
      Abstract
    • , 'Fermentation, cell culture, bioprocessing: Digoxin production by using biotransformation in Digitalis lanata cell suspension cultures'
      Korean Journal of Microbiology and Biotechnology 22 (6) (1994) pp.651-658
      ISSN: 1598-642X eISSN: 1598-642X

    Book chapters

    • Hong HJ, Hutchings MI, Buttner MJ, 'Vancomycin resistance VanS/VanR two-component systems' in Utsumi R (ed.), Vancomycin resistance VanS/VanR two-component systems, Kluwer Academic Publishers (2008)
      ISBN: 978-0-387-78884-5
      Abstract Website

    Other publications

    Peer reviewed - journal

    1. Zarkan, A., Macklyne, H.-R., Chirgadze, D.Y., Bond, A.D., Hesketh, A.R., and Hong, H.-J. (2017) Zn(II) mediates vancomycin polymerization and potentiates its antibiotic activity against resistant bacteria. Sci. Rep. Accepted.

    2. Lee, D.-R., Cheng, J., Lee, S.-K., Hong, H.-J., Song, J., Yang, S.H., and Suh, J.-W. (2016) Anti-multi drug resistant pathogen activity of siderochelin A, produced by a novel Amycolatopsis sp. KCTC 29142. Korean J. Microbiol. 52:327-345.

    3. Hong, H.-J. (2016) Construction of a bioassay system to identify extracellular agents targeting bacterial cell envelope. Methods Mol Biol. 1440:125-137.

    4. Hesketh, A., and Hong, H.-J. (2016) Microarray analysis to monitor bacterial cell wall homeostasis. Methods Mol Biol. 1440:31-46.

    5. Zarkan, A., Macklyne, H.-R., Truman, A.W., Hesketh, A.R., and Hong, H.-J. (2016) The frontline antibiotic vancomycin induces a zinc starvation response in bacteria by binding to zinc(II). Sci. Rep. 6:19602.

    6. Novotna, G.B., Kwun, M.J., and Hong, H.-J. (2015) In vivo characterization of the activation and interaction of the VanR/VanS two-component regulatory system controlling glycopeptide antibiotic resistance in two related Streptomyces species. Antimicrob. Agents Chemother. 60:1627-1637.

    7. Cha, J.-Y., Han, S., Hong, H.-J., Cho, H., Kim, D., Kwon, Y., Kwon, S.-K., Crüsemann, M., Lee, Y.B., Kim, J.F., Giaever, G., Nislow, C., Moore, B.S., Thomashow, L.S., Weller, D.M., and Kwak, Y.-S. (2015) Microbial and biochemical basis of a Fusarium wilt suppressive soil. ISME J. In press.

    8. Hesketh, A., Deery, M., Hong, H.-J. (2015) High-resolution mass spectrometry based proteomic analysis of the response to vancomycin-induced cell wall stress in Streptomyces coelicolor A3(2). J Proteome Res.14:2915-2928.

    9. Kwun, M.J., Cheng, J., Yang, S.H., Lee, D.R., Shu, J.-W., and Hong, H.-J. (2014) Draft genome sequence of ristocetin-producing strain Amycolatopsis sp. Strain MJM2582 isolated in South Korea. Genome Announc. 2:e01091-14.

    10. Kwun, M.J., and Hong, H.-J. (2014) Draft genome sequence of Amycolatopsis lurida NRRL 2430, producer of the glycopeptide family antibiotic ristocetin. Genome Announc. 2:e01050-14.

    11. Kwun, M.J., and Hong, H.-J. (2014) The activity of glycopeptide antibiotics against resistant bacteria correlates with their ability to induce the resistance system. Antimicrob. Agents Chemother. 58:6303-6310.

    12. Kwun, M.J., and Hong, H.-J. (2014) Genome sequence of Streptomyces toyocaensis NRRL 15009, producer of the glycopeptide antibiotic A47934. Genome Announc. 2:e00749-14.

    13. Truman, A.W., Kwun, M.J., Cheng, J., Yang, S.H., Shu, J.-W., and Hong, H.-J. (2014) Antibiotic resistance mechanisms inform discovery: Identification and characterization of a novel Amycolatopsis strain producing Ristocetin. Antimicrob. Agents Chemother. 58:5687-5695.

    14. Hesketh, A.R., and Hong, H.-J. (2013) Comparative and functional genomics as tools for understanding vancomycin resistance in bacteria. Nova Science Publishers, New York.

    15. Kwun, M.J., Novotna, G., Hesketh, A.R., Hill, L., and Hong, H.-J. (2013) Induction of VanS-dependent vancomycin resistance requires binding of the drug to D-Ala-D-Ala termini in the peptidoglycan cell wall. Antimicrob. Agents Chemother. 57:4470-4480.

    16. Duong, A., Capstick, D.S., Berardo, C.D., Findlay, K., Hesketh, A., Hong, H.-J., and Elliot, M.A. (2012) Aerial development in Streptomyces coelicolor requires sortase activity. Mol Microbiol. 83:992-1005.

    17. Novotna, G., Vincent, K., Hill, C., Liu, C., and Hong, H.-J. (2012) A novel membrane protein, VanJ, conferring resistance to teicoplanin. Antimicrob. Agents Chemother. 56:1784-1796.

    18. Hesketh, A., Hill, C., Mokhtar, J, Novotna, G., Tran, N., Bibb, M., and Hong, H.-J. (2011) Genome-wide dynamics of a bacterial response to antibiotics that target the cell envelope. BMC Genomics. 12:226.

    19. Hong, H.-J. (2010) Studying gene induction of glycopeptide resistance using gene swapping. Methods Mol Biol. 642:45-62.

    20. Koteva, K., Hong, H.-J., Wang, X.D., Nazi, I., Hughes, D., Naldrett, M.J., Butter, M.J., and Wright, G.D. (2010) A vancomycin photoprobe identifies the histidine kinase VanSsc as a vancomycin receptor. Nat Chem Biol. 6:327-329.

    21. Kallifidas, D., Pascoe, B., Owen, G.A., Strain-Damerell, C.M., Hong, H.-J., and Paget, M.S. (2010) The zinc-responsive regulator Zur controls expression of the coelibactin gene cluster in Streptomyces coelicolor. J Bacteriol. 192:608-611.

    22. Hong, H.-J., Hutchings, M.I., and Buttner, M.J. (2008) Vancomycin resistance VanRS two-component systems. Adv Exp Med Biol, 631:200-213.

    23. Hutchings, M.I., Hong, H.-J., Leibovitz, E., Sutcliffe, I.C., and Buttner, M.J. (2006) The SigE cell envelope stress signal of Streptomyces coelicolor is influenced by a novel lipoprotein, CseA. J Bacteriol. 188(20):7222-7229.

    24. Hutchings, M.I., Hong, H.-J., and Buttner M.J. (2006) The vancomycin resistance VanRS signal transduction system of Streptomyces coelicolor. Mol. Microbiol. 59(3):923-935. (Joint first author)

    25. Hong, H.-J., Hutchings, M.I., Hill, L., and Buttner, M.J. (2005) The role of the novel Fem protein VanK in vancomycin resistance in Streptomyces coelicolor J. Biol. Chem. 280(13):13055-13061. (First and corresponding author)

    26. Hong, H.-J., Hutchings, M.I., Neu, J.M., Wright G.D., and Buttner, M.J. (2004) Characterization of an inducible vancomycin resistance system in Streptomyces coelicolor reveals a novel gene (vanK) required for drug resistance. Mol. Microbiol. 52(4):1107-1121. (First and corresponding author)

    27. Hong, H.-J., Paget, M.S.B., and Buttner, M.J. (2002) A signal transduction system in Streptomyces coelicolor that activated expression of a putative cell wall glycan operon in response to vancomycin and other cell wall-specific antibiotics. Mol. Microbiol. 44(5):1199-1211. (First and corresponding author)

    28. Hong, H.-J., Paget, M.S.B., Hutchings, M.I., Leibovitz, E., and Buttner, M.J. (2002) Sensing and responding to cell envelope stress in Streptomyces coelicolor. Actinomycetol. 16(2):41-47. (Review Article)

    29. Bibb, M.J., Blomfield, I.C., Browning, D., Buck, M., Busby, S., Buttner, M.J., Dixon, R., Dorman, C.J., Finn, R.D., Gerdes, K., Green, J., Harris, A.K.P., van Heel, M., Henkin, T.M., Hoch, J.A., Hong, H.-J., Hood, D., Lee, D., Møller-Jensen, J., Morrison, D.A., Moxon, R., Müller-Hill, B., Neidhardt, F.C., Orlova, E.V., Paget, M.S.B., Salmond, G.P.C., Schleif, R., Whitehead, N.A., and Williams, P. (2002) Signal, switches, regulons and cascades: control of bacterial gene expression. Hodgson, D.A., and Thomas, C.M. (eds). Cambridge:Unversity Press.

    30. Kim, E.-S., Hong, H.-J., Choi, C.-Y., and Cohen, S.N. (2001) Modulation of actinorhodin biosynthesis in Streptomyces lividans by glucose repression of afsR2 gene transcription. J Bacteriol. 183(7):2198-2203. (Joint first author)

    31. Hong, H.-J., Lee J.-E., Ahn, J.-E., and Kim, D.-I. (1998) Enhanced Production of Digoxin by Digitoxin Biotransformation Using In Situ Adsorption in Digitalis lanata Cell Culture. J. Microbiol. Biotechnol. 8(5):478-483.

    32. Park, Y.-S., Kim, H.-K., Hong, H.-J., and Kim, D.-I. (1995) Biotransformation using immobilized cell culture of Digitalis lanata. J. of Korean Ind. & Eng. Chemistry 6(2):222-229.

    33. Kim, H.-K., Hong, H.-J., and Kim, D.-I. (1994) Fermentation, cell culture, bioprocessing: Digoxin production by using biotransformation in Digitalis lanata cell suspension cultures. Korean Journal of Applied Microbiology and Biotechnology 22(6):651-658.


    Peer reviewed - Conference proceedings

    1. Hong, H.-J. (2015) Antibiotics for the future: Reistance informs discovery. 2015 International Meeting of the Microbiological Society of Korea, Changwon, South Korea, p 49.

    2. Kwun M.J., Novotna, G., Hesketh, A.R., Hill, L., and Hong, H.-J. (2013) Characterisation of the effector ligand recognised by VanS, a key protein triggering resistance to vancomycin. 16th International Symposium on the Genetics of Industrial Microorganisms, Cancun, Mexico, p 43.

    3. Hong, H.-J. (2013) Genome-wide dynamics for bacterial cell response to antibiotics, 1st China-Europe Symposium “The Biology of Actinomycete Antibiotic Production”, Beijing, China, p 31.

    4. Kwun M.J., Novotna, G., Hesketh, A.R., Hill, L., and Hong, H.-J. (2013) Induction of VanS-dependent vancomycin resistance requires binding of the drug to D-Ala-D-Ala termini in the peptidoglycan cell wall. Society for General Microbiology, Manchester, UK, p 68.

    5. Al Zarkan, M.A., Heather M., and Hong, H.-J. (2013) Vancomycin incudes the zinc starvation response in S. coelicolor. Society for General Microbiology, Manchester, UK, p 68.

    6. Liu, W., and Hong, H.-J. (2013) Characterisation of a novel membrane protein VanJ conferring resistance to teicoplanin. Society for General Microbiology, Manchester, UK, p 112.

    7. Novotna, G., Hill, C., Vincent, K., Liu, C., and Hong, H.-J. (2011) A novel membrane protein, VanJ, conferring resistance to teicoplanin. 16th International Symposium on the Biology of Actinomycetes, Vuerto Vallarta, Mexico, p 97.

    8. Novotna, G., Hill, C., Vicent, K., Liu, C., and Hong, H.-J. (2011) A novel membrane protein, VanJ, conferring resistance to teicoplanin. 2nd Great Wall Symposium - The Dynamics of Peptidoglycan Structure and Function : New Insights into The ‘Great Wall’. Cascais, Portugal, p 73.

    9. Novotna, G., Hill, C., Vincent, K., and Hong, H.-J. (2011) Glycopeptide resistance: Old and New. 2nd International Symposium of the Collaborative Research Centre 766 - The Bacterial Envelope: Structure, Function, and Infection Interface, Function, and Infection Interface at Kloster Irsee conference centre. Kaufbeuren, Germany, p 83.

    10. Novotna, G., and Hong, H.-J. (2010) VanJ – A novel resistance to glycopeptides. UKCan-BaCWAN Meeting,White Oaks Conference Resort & Spa, Niagara-on-the-lake, Ontario, Canada, p 27.

    11. Novotna, G., Ulanova, D., and Hong, H.-J. (2009) Glycopeptide resistance VanS/VanR two-component wystems, UK-BaCWAN Meeting, Woodland Grange conference centre, Leamington Spa, Warwichkshire, UK, p 36.

    12. Hong, H.-J. (2009) Streptomyces coelicolor as a model system for characterising resistance to antibiotics that target the bacterial cell wall. Society for General Microbiology, Edinburgh Conference Centre, Herriot-Watt University, UK, p 31.
    13. Novotna, G., Ulanova, D., and Hong, H.-J. (2009) What induces resistance to glycopeptide antibiotics? 15th International Symposium on the Biology of Actinomycetes, Shanghai, China, p 17.

    14. Novotna, G., Mitchell, H., and Hong, H.-J. (2009) Glycopeptide resistance VanS/VanR two-component wystems. 15th International Symposium on the Biology of Actinomycetes, Shanghai, China, p 103.

    15. Hong, H.-J. (2009) Bacterial cell wall homeostasis. Seoul International Conference on Actinomycetes, Seoul National University, Seoul, South Korea, p 5.

    16. Hong, H.-J. (2008) Streptomyces coelicolor as a model system for characterising resistance to antibiotics that target the bacterial cell wall. Japan-UK Workshop on Genomics of Antibiotic producing Actinomycetes: Implications and Applications. Nihon University, Tokyo, Japan, p 28.

    17. Hong, H.-J., Hutchings, M., and Buttner, M.J. (2007) Inducible vancomycin resistance in bacteria. UK-BaCWAN Meeting, Honiley court Hotel & Conference centre, West midlands, UK.

    18. Hong, H.-J. (2006) Characterization of bacterial responses to cell wall-specific antibiotic attack. Korean Streptomyces meeting. Han-Hwa Resort, Yong-In, South Korea.

    19. Hong, H.-J. (2006) Outsmarting the vancomycin resistance system. UK-BaCWAN Meeting, Warwick Biological Sciences, University of Warwick, UK.

    20. Hong, H.-J. (2006) Sneaking past the VanS vanguard: Outsmarting the vancomycin resistance system. UK Streptomyces Community Dissemination Meeting, John Innes Centre, Norwich, UK.

    21. Hong, H.-J., and Buttner, M.J. (2005) The role of novel Fem protein VanK in vancomycin resistance in Streptomyces coelicolor. International Union of Microbiological Societies meeting, San Francisco, USA, p 106.

    22. Hong, H.-J., and Buttner, M.J. (2005) Novel vancomycin resistance system in Streptomyces coelicolor. International meeting of the microbiological society of Korea, Hundai Sungwoo Resort, Gangwondo, South Korea, p 143-147.

    23. Hong, H.-J., Hutchings, M.I., Hill, L., and Buttner, M.J. (2005) The role of novel Fem protein VanK in vancomycin resistance in Streptomyces coelicolor. 5th International symposium on antimicrobial agents and resistance, COEX Center, Seoul, South Korea.
    24. Hong, H.-J., Hutchings, M.I., and Buttner, M.J. (2005) The function of novel gene vanK for vancomycin resistance system in Streptomyces coelicolor. Microbes in Norwich, UK.

    25. Hong, H.-J., Hutchings, M.I., and Buttner, M.J. (2005) The role of the novel Fem protein VanK in vancomycin resistance in Streptomyces coelicolor. UK Streptomyces Community Dissemination Meeting, University of Surrey, Guildford, UK, p 45.

    26. Hong, H.-J. (2004) Superbug threat!: Are we living in the “Era of Phamageddon?”. 29th Annual Meeting of Korean Scientists and Engineers’ Association in the UK for 30th anniversary of KSEAUK, University of College London, UK.

    27. Hong, H.-J., Hutchings, M.I., and Buttner, M.J. (2003) Inducible cell wall remodelling conferring vancomycin resistance in Streptomcyes coelicolor. 13th International Symposium on the Biology of Actinomycetes, Melbourne, Australia, p 26.

    28. Hong, H.-J., and Buttner, M.J. (2003) Pharmageddon: the spread of inducible vancomycin resistance in bacteria. Norwich Research Park Symposium “Drugs and Bugs”, Institute of Food Research Lecture Theatre, Norwich, UK.

    29. Hong, H.-J., Hutchings, M.I., and Buttner, M.J. (2003) The Vancomycin resistance system in Strpetomyces coelicolor: identification of two novel genes. UK Streptomyces Community Dissemination Meeting, University of Surrey, Guildford, UK.

    30. Hong, H.-J., Hutchings, M.I., and Buttner, M.J. (2003) Novel vancomycin resistance gene cluster in Streptomyces coelicolor. Microbes in Morwich, UK.

    31. Hong, H.-J. (2002) Induction of vancomycin resistance in bacteria – how cell walls talk to the chromosome. Norwich Research Park Symposium “Signalling and Communication”, Institute of Food Research Lecture Theatre, Norwich, UK.

    32. Hong, H.-J., Hutchings, M.I., and Buttner, M.J. (2002) Novel vancomycin-resistance gene cluster in Streptomyces coelicolor. JIC Annual Science Meeting, Norwich, UK.

    33. Hong, H.-J., Hutchings, M.I., and Buttner, M.J. (2002) Novel vancomycin resistance gene cluster in Streptomyces coelicolor. 9th International Symposium on the Genetic on Industrial Microorganisms, Gyeonju, South Korea, p 90.

    34. Paget, M.S.B., Hong, H.-J., Bibb, M.J., and Buttner, M.J. (2002) The ECF sigma factors of Streptomcyes coelicolor A(3)2. 150th Society for General Microbiology, University of Warwick, UK.

    35. Hong, H.-J., and Buttner, M.J. (2002) Characterisation of a vancomycin resistance system in Streptomyces coelicolor. Microbes in Norwich, UK.

    36. Hong, H.-J., and Buttner, M.J. (2001) Characterisation of a vancomycin resistance system in Streptomyces coelicolor. JIC Annual Science Meeting, Norwich, UK.

    37. Hong, H.-J., Paget, M.S.B., Leibovitz, E., and Buttner, M.J. (2001) Sensing and responding to cell envelope stress in Streptomyces coelicolor, 12th International Symposium on the Biology of Actinomycetes, Vancouver, Canada, p 122. 38. Hong, H.-J., Paget, M.S.B., and Buttner, M.J. (2001) Sensing and responding to cell envelope stress in Streptomyces. 13th RNA Polymerase Workshop, Nottingham, UK.

    39. Hong, H.-J. (2000) Sensing and responding to cell envelope stress in Streptomyces coelicolor. The 26th Annual Meeting of Korean Scientists and Engineers’ association in the UK, Oxford, UK, p 16.

    40. Hong, H.-J., and Buttner, M.J. (2000) A signal transduction system in Streptomyces coelicolor that activates cell wall glycan biosynthesis in response to vancomycin and other cell wall-specific antibiotics. JIC Annual Science Meeting, Norwich, UK.

    41. Kim, D.I., Hong, H.-J., Lee, J.E., and Choi, Y.S. (1997) Extractive bioconversion using solid adsorbent in plant cell suspension cultures. 4th Asia-Pacific Biochemical Engineering Conference, Central Garden Hotel, Beijing, China.

    42. Hong, H.-J., Lee, J.E., Choi, Y.S., and Kim, D.I. (1997) Integrated bioprocessing for enhanced production of digoxin in Digitalis lanata suspension cell cultures. 2nd Inha-Wuxi Biotechnology Symposium, Inha University, South Korea.

    43. Kim, D.I., Hong, H.-J., and Lee. J.E. (1995) Enhanced bioconversion of digitoxin to digoxin using in situ adsorption in plant cell cultures. International Chemical Congress of Pacific Basin Societies (Pacifichem'95), Hilton Hotel, Honolulu, Hawaii, USA.

    44. Hwang, Y.S., Hong, H.-J., and Kim, D.I. (1995) Process development for de novo biosynthesis and biotransformation usin plant cell cultures. International Symposium on Functional Biomaterials, Seoul National University Cultural Center, Seoul, South Korea.

    45. Kim, H.K., Park, Y.S., Hong, H.-J., and Kim, D.I. (1995) Process strategy for biotransformation of digitoxin to digoxin by plant cell cultures. 7th European Congress on Biotechnology, Nice, France.

     

    Patents

    1. Hong, H.-J., Hutchings, M.I., Buttner, M.J. (WO 2005001118). Detection system: a patent application covering the use of femX mutant bioassay as the basis of a broad-range, generic screening for cell envelope-specific antibiotics was filed through Plant Bioscience Ltd.

    2. Hong, H.-J., Paget, M.S.B., Buttner, M.J. (US 2004014066) Screening system: a patent application covering the use of a vanJ promoter-reporter gene bioassay as the basis of a broad-range, generic screening for cell envelope-specific antibiotics was filed through Plant Bioscience Ltd.

    3. Hong, H.-J., Paget, M.S.B., Buttner, M.J. (WO 03012128) Detection system: a patent application covering the use of a sigE promoter-reporter gene bioassay as the basis of a broad-range, generic screening for cell envelope-specific antibiotics was filed through Plant Bioscience Ltd.

     

    Areas of expertise

    Bacterial Cell Wall Homeostasis Antimicrobial Resistance and Development Bacterial Functional Genomics Actinomycetes molecular microbiology

    Membership of professional bodies

    Member of Society of General Microbiology (SGM)
    American Society for Microbiology (ASM)
    Member of The Microbiology Society of Korea (MSK)
    Member of The Korean Scientists and Engineers Association in the UK (KSEAUK)
    Editor of the book edition on 'Bacterial Cell Wall Homeostasis' in the Methods in Molecular Biology series, Springer, Humana Press 
    Scientific advisory board member of UC-CARE (University of Copenhagen Research Centre for Control of Antibiotic Resistance 
    Before I came to Oxford as a senior lecturer in January 2016, I was working in the Department of Biochemistry at the University of Cambridge with the award of an independent research fellowship from the Royal Society of London in 2006. I also secured a three year MRC New Investigator grant in 2007 providing funds for a post-doctoral researcher, and for equipment to establish my research group. My group in Cambridge had expertise in molecular microbiology and a proven record in elucidating the mechanisms of bacterial antibiotic resistance. I have also been developing collaborative links with world-class research groups in the UK, Germany, Denmark, France, US, Canada, Japan and South Korea. The outcome of the international collaboration was particularly fruitful as it enabled us to publish several interesting papers and to form a research consortium grant application. I have also been involved in various undergraduate/postgraduate teachings in the Department of Biochemistry. I have run a microbial biochemistry practical course for second year students and have given two core lectures for third year students, one entitled ‘Pathogenic Bacteria: Signalling and Secretion’ in the ‘Biochemistry of Microorganisms’ course, and the other entitled ‘Anatomy of an Infection’ as part of the ‘Molecular Microbiology of Infectious Disease’ course. I have also been running one of the core journal clubs for second year students under a molecular microbiology subject each year, and have acted as an examiner of the Molecules in Medical Science (MIMS) Part I-A (1st year). In addition, I have been annually accommodating and supervising third and fourth year project students in my group, and have been involved in various small-group undergraduate tutorials and examination processes. Over the last 10 years I have supervised 12 graduate students, 7 international visiting students and a post-doctoral research associate.

    Other publicity

     
    During my time in Cambridge, I have been involved in some external activities toward public engagement and school education. I was a leading organizer of the Departmental Science Day exhibition as part of the “800 Years of Cambridge Science Festival” in March 2009. I have also been involved in helping to run school visiting days to the Department, and the 2013 Departmental Science day. This year, I am also involved in a School Project entitled ‘Antibiotic Unearthed’ sponsored by Microbiology Society. It is to work on a project to find new antibiotics in soil via established partnership with a school and I am currently conducting the project with UTC Oxfordshire. For more detail of this project, visit links below.
     
    https://www.microbiologysociety.org/education-outreach/antibiotics-unearthed.html

    https://www.microbiologysociety.org/education-outreach/antibiotics-unearthed/antibiotics-and-antibiotic-resistance.html