Department of Biological and Medical Sciences


  • Our published research articles

    Hitchman RB, Possee RD, King LA. 2012. High-throughput baculovirus expression in insect cells. Methods in Molecular Biology, 824, 609-627.

    Bak A, Irons SL, Martinière A, Blanc S, Drucker M. 2012. Host cell processes to accomplish mechanical and non-circulative virus transmission. Protoplasma. 249, 529-539.

    Danquah JO, Botchway S, Jeshtadi A, King LA. 2012. Direct interaction of baculovirus capsid proteins VP39 and EXON0 with kinesin-1 in insect cells determined by fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy. Journal of Virology. 86, 844-853.

    Hitchman RB, Locanto E, Possee RD, King LA. 2011. Optimizing the baculovirus expression vector system. Methods. 55, 52-57.

    Hitchman RB, Murguía-Meca F, Locanto E, Danquah J, King LA. 2011. Baculovirus as vectors for human cells and applications in organ transplantation. Journal of Invertebrate Pathology. 107, S49-58.

    van Oers MM, King LA. The application of baculoviruses in human and veterinary medicine: an overview. Preface. Journal of Invertebrate Pathology. 107. S1-2.

    Murguía-Meca F, Plata-Muñoz JJ, Hitchman RB, Danquah JO, Hughes D, Friend PJ, Fuggle SV, King LA. 2011. Baculovirus as delivery system for gene transfer during hypothermic organ preservation. Transplant International 24, 820-828.

    Hitchman, R. B., Possee, R. D., Crombie, A. T., Chambers, A., Ho, K., Siaterli, E., Lissina, O., Sternard, H., Novy, R., Loomis, K., Bird, L. E., Owens, R. J. & King, L. A. 2010. Genetic modification of a baculovirus vector for increased expression in insect cells. Cell Biology and Toxicology, 26, 57-68.

    Hitchman, R. B., Possee, R. D., Siaterli, E., Richards, K. S., Clayton, A. J., Birds, L. E., Owens, R. J., Carpentier, D. C. J., King, F. L., Danquah, J. O., Spink, K. G. & King, L. A. 2010. Improved expression of secreted and membrane-targeted proteins in insect cells. Biotechnology and Applied Biochemistry, 56, 85-93.

    Jeshtadi, A., Burgos, P., Stubbs, C. D., Parker, A. W., King, L. A., Skinner, M. A. & Botchway, S. W. 2010. Interaction of Poxvirus Intracellular Mature Virion Proteins with the TPR Domain of Kinesin Light Chain in Live Infected Cells Revealed by Two-Photon-Induced Fluorescence Resonance Energy Transfer Fluorescence Lifetime Imaging Microscopy. Journal of Virology, 84, 12886-12894.

    Birks, S., Danquah, J., King, L., Gorecki, D. & Pilkington, G. 2010. GD3 AS A POTENTIAL THERAPEUTIC TARGET IN GLIOMA. Neuro-Oncology, 12, 3-3.

    Hitchman, R. B., Possee, R. D. & King, L. A. 2009. Baculovirus expression systems for recombinant protein production in insect cells.  Recent Pat. Biotechnol., 3(1), 46-54.

    Carpentier, D. C. J. & King, L. A. 2009. The long road to understanding the baculovirus P10 protein. Invited Review: Virolgica Sinica, 24, 227-242. DOI: 10.1007/s12250-009-3045-0.

    Haines, F. J., Griffiths, C. M., Possee, R. D., Hawes, C. R. & King, L. A. 2009. Involvement of lipid rafts and cellular actin in ACMNPV GP64 distribution and virus budding. Virologica Sinica, 24, 333-349. DOI: 10.1007/s12250-009-3055-y.

    Birks, S., Danquah, J., Hitchman, R., King, L., Vlasak, R., Gorecki, D. & Pilkington, G. J. 2008. Manipulation Of Glioma Cell Surface Gangliosides: Potential Therapeutic Implications. Anticancer Research, 28, 60.

    Carpentier, D. C. J., Griffiths, C. M. & King, L. A. 2008. The baculovirus P10 protein of Autographa californica qucleopolyhedrovirus forms two distinct cytoskeletal-like structures and associates with polyhedral occlusion bodies during infection. Virology, 371, 278-291.

    Kelly, B. J., Chapple, S. D. J., Allen, C., Pritchard, C., King, L. A. & Possee, R. D. 2008. Extended budded virus formation and induction of apoptosis by an AcMNPV FP-25/p35 double mutant in Trichoplusia ni cells. Virus Research, 133, 157-166.

    Murguia-Meca, F., Hitchman, R. B., Plata-Munoz, J. J., Friend, P. J. & King, L. A. 2008. A novel baculovirus delivery tool for gene therapy in in vitro and ex vivo models of renal ischemic injury. American Journal of Transplantation, 8, 633-633.

    Possee, R. D., Hitchman, R. B., Richards, K. S., Mann, S. G., Siaterli, E., Nixon, C. P., Irving, H., Assenberg, R., Alderton, D., Owens, R. J. & King, L. A. 2008. Generation of Baculovirus Vectors for the High-Throughput Production of Proteins in Insect Cells. Biotechnology and Bioengineering, 101, 1115-1122.

    Hitchman, R., Siaterli, E., Nixon, C. and King, L.A. 2007. Quantitative real-time PCR for rapid and accurate titration of recombinant baculovirus particles. Biotechnol. Bioeng., 96, 810-814.

    Hitchman, R., Hodgson, D., King, L.A., Cory, J.S., Hails, R. and Possee, R.D. 2007. Host mediated selection of pathogen genotypes as a mechanism for the maintenance of baculovirus diversity in the field. J. Invert. Pathol., 94, 153-162.

    Burden, J., Possee, R., Sait, S., King, L.A. and Hails, R. 2006. Phenotypic and genotypic characterisation of persistent baculovirus infections in populations of the cabbage moth (Mamestra brassicae) within the British Isles. Arch Virol., 151, 635-49.

    Kelly, B., King, L.A., Possee, R. and Chapple, S. 2006. Dual mutations in the Autographa californica nucleopolyhedrovirus FP-25 and p35 genes result in plasma-membrane blebbing in Trichoplusia ni cells. J Gen Virol., 87, 531-6.

    Burden, J., Nixon, C., Hodgekinson, A., Possee, R.D., Sait, S., King, L.A. and Hails, R. 2005. Covert infections as a mechanism for the long-term persistence of baculoviruses. Ecology Letters, 6, 524-531.

    Saville, G.P., Patmanidi, A.L., Possee, R.D. and King, L.A. 2004. Deletion of the Autographa californica nucleopolyhedrovirus chitinase KDEL motif and in vitro and in vivo analysis of the modified virus. Journal of General Virology, 85, 821-831.

    Burden, J.P., Nixon, C.P., Hodgkinson, A.E., Possee, R.D., Sait, S.M., King, L.A. and Hails, R.S. 2003. Covert infections as a mechanism for long-term persistence of baculoviruses. Ecology Letters, 6, 524-531.

    Patmanidi, A.L., Possee, R.D. and King, L.A. 2003. Formation of P10 tubular structures during AcMNPV infection depends on the integrity of host-cell microtubules. Virology, 317, 308-320.

    Saville, G.P., Thomas, C.J., Possee, R.D. and King, L.A. 2002. Partial redistribution of the Autographa californica nucleopolyhedrovirus chitinase in virus-infected cells accompanies mutation of the carboxy-terminal KDEL ER-retention motif. Journal of General Virology, 83, 685-694.

    Grasela, J.J., McIntosh, A.H., Goodman, C.L., Wilson, L.E. and King, L.A. 2000. Expression of the green fluorescent protein carried by Autographa Californica multiple nucleopolyhedrovirus in insect cell lines In Vitro. Cell. Dev. Biol. - Animal, 36, 205-210. 

    Lampitt, R.S., Newton, P.P., Jickells, T.D., Thomson, J. and King, L.A. 2000. Near-bottom particle flux in the abyssal northeast Atlantic. Deep-Sea Research II, 47, 2051-2071. 

    Thomas, C.J., Gooday, G.W., King, L.A. and Possee, R.D. 2000. Mutagenesis of the active site coding region of the Autographa californica nucleopolyhedrovirus chiA gene. Journal of General Virology, 81, 1403-1411. 

    Shi, J., Thomas, C.J., King, L.A., Hawes, C.R., Possee, R.D., Edwards, M.L., Pallett, D. and Cooper, J.I. 2000. The expression of a baculovirus-derived chitinase gene increased resistance of tobacco cultivars to brown spot (Alternaria alternata). Annals of Applied Biology, 136, 1-8. 

    Griffiths, C.M., Barnett, A.L., Ayres, M.D., Windass, J., King, L.A. and Possee, R.D. 1999. In vitro host range of Autographa californica nucleopolyhedrovirus recombinants lacking functional p35, iap1 or iap2. Journal of General Virology, 80, 1055-1066. 

    King, L.A., Thomas, C.J., Wilkinson, N. and Possee, R.D. 1999. Expression of green fluorescent protein using baculovirus vectors. Green Fluorescent Protein, 302, 394-408. 

    Possee, R.D., Thomas, C.J. and King, L.A. 1999. The use of baculovirus vectors for the production of membrane proteins in insect cells. Biochemical Society Transactions, 27, 928-932. 

    Marlow, S.A., Wilson, L.E., Lawrie, A.M., Wilkinson, N. and King, L.A. 1998. Assembly of Amsacta moorei entomopoxvirus spheroidin into spheroids following synthesis in insect cells using a baculovirus vector. Journal of General Virology, 79, 623-628. 

    Thomas, C.J., Brown, H.L., Hawes, C.R., Lee, B.Y., Min, M.K., King, L.A. and Possee, R.D. 1998. Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum. Journal of Virology, 72,10207-10212. 

    Aspinwall, L.S., Bermudez, I., King, L.A. and Wafford, K.A. 1997. The interactions of hexachlorocyclohexane isomers with human gamma- aminobutyric acid(A) receptors expressed in Xenopus oocytes. Journal of Pharmacology and Experimental Therapeutics, 282, 1557-1564. 

    Hughes, D.S., Possee, R.D. and King, L.A. 1997. Evidence for the presence of a low-level, persistent baculovirus infection of Mamestra brassicae insects. Journal of General Virology. 78, 1801-1805. 

    McCarroll, L. and King, L.A. 1997. Stable insect cell cultures for recombinant protein production. Current Opinion in Biotechnology, 8, 590-594. 

    Possee, R.D., Barnett, A.L., Hawtin, R.E. and King, L.A. 1997. Engineered baculoviruses for pest control. Pesticide Science, 51, 462-470. 

    Watkins, M.J., Kiyatkin, N., Hughes, D., Beadle, D.J. and King, L.A. 1997. Study on the biological properties of a novel recombinant baculovirus. BCPC Monograph, Microbial Insecticides: Novelty or Necessity?, 68, 279-284. 

    Wilson, L.E., Wilkinson, N., Marlow, S.A., Possee, R.D. and King, L.A. 1997. Identification of recombinant baculoviruses using green fluorescent protein as a selectable marker. Biotechniques,  22:674. 

    Obosi, L.A., Hen, R., Beadle, D.J., Bermudez, I. and King, L.A. 1997. Mutational analysis of the mouse 5-HT7 receptor: importance of the third intracellular loop for receptor-G-protein interaction. Febs Letters, 412, 321-324. 

    Hawtin, R.E., Zarkowska, T., Arnold, K., Thomas, C.J., Gooday, G.W., King, L.A., Kuzio, J.A. and Possee, R.D. 1997. Liquefaction of Autographa californica nucleopolyhedrovirus-infected insects is dependent on the integrity of virus-encoded chitinase and cathepsin genes. Virology, 238, 243-253. 

    Henderson, J., Napier, R.M., Lazarus, C.M. and King, L.A. 1996. Transgene expression in insect cells: Comparison of transformation and baculovirus infection. Molecular Biology of the Cell, 7, 448-448. 

    Obosi, L.A., Schuette, D.G., Europe Finner, G.N., Beadle. D.J., Hen, R., King, L.A. and Bermudez, I. 1996. Functional-Characterization of the Drosophila 5-HTdro1 and 5-HTdro2b Serotonin Receptors in Insect Cells - Activation of a G(Alpha-S)-Like Protein By 5-HTdro1 But Lack of Coupling to Inhibitory G-Proteins by 5-HTdro2b. Febs Letters, 381, 233-236. 

    Atkinson, A.E., Henderson, J., Hawes, C.R. and King, L.A. 1996. Efficient membrane targeting of the chick nicotinic acetylcholine- receptor alpha-subunit in stable insect-cell lines. Cytotechnology, 19, 37-42. 

    Merrington, C.L., Kitts, P.A., King, L.A. and Possee, R.D. 1996. An Autographa californica nucleopolyhedrovirus lef-2 mutant: Consequences for DNA replication and very late gene expression. Virology, 217, 338-348.

    Review articles

    Haines, F.J., Possee, R.D. and King, L.A. 2007. Baculovirus Expression Vectors. In: Encyclopaedia of Virology, in press.

    King, L.A. 2006. Baculovirus Biotechnology. In: Public Service Review: Trade and Industry, 10, 50-52.

    King, L.A. and Possee, R. 1997. A future for baculovirus insecticides? Focus on Biopesticides Plus. The Royal Society of Chemistry, 1-2.

    Book Chapters

    Hitchman, R.B., Possee, R.D. and King, L.A. 2007. Improved Baculovirus Expression Vectors. In: Methods Express: Expression Systems, Eds. Dyson, MR and Durocher, Y pp. 147-167. Scion Publishing Ltd, Oxford.

    Hitchman, R., Possee, R.D. and King, L.A. 2007. Recombinant baculovirus isolation. In: Methods in Molecular Biology 338: Baculovirus and Insect Cell Expression Protocols 2nd Edition. Ed. Murhammer, DW. pp. 77-93. Humana Press Inc. NJ.

    Kelly, B.J., King, L.A. and Possee, R.D. 2007. Introduction to baculovirus molecular biology. In: Methods in Molecular Biology 338: Baculovirus and Insect Cell Expression Protocols 2nd Edition. Ed. Murhammer, DW. pp. 25-55. Humana Press Inc. NJ.

    King, L.A. and Possee, R.D. 2007. Baculovirus transfer vectors. In: Methods in Molecular Biology 338: Baculovirus and Insect Cell Expression Protocols 2nd Edition. Ed. Murhammer, DW. pp. 55-75. Humana Press Inc. NJ.

    Merrington, C.L., King, L.A. and Possee, R.D. 1999. Baculovirus Expression Systems. In S. J. a. H. Higgins, B.D. (ed.), Protein Expression, A Practical Approach. Oxford University Press, Oxford. 

    King, L.A., MArlow, S.A., Obosi, L.A. and Possee, R.D. 1998. The Baculovirus Expression Vector systems II: Amplification and Characterization of Recombinant Viruses Cell Biology Handbook 2nd Edn. (Ed. J. Celis). Academic Press:211-217. 

    King, L.A., Marlow, S.A., Wilson, L.E. and Possee, R.D. 1998. The Baculovirus expression vector system I: Production and Isolation of Recombinant Viruses Cell Biology Handbook. Ed K. Celis. Academic Press, New York:204-210. 

    King, L.A., Wilkinson, N., Miller, G. and Marlow, S. 1998. Entomopoxviruses: The Insect Viruses, p. 1-29. In L. K. M. a. L. A. Ball (ed.). Plenum Pres, New York. 

    King, L.A., Possee, R. et. al. 1998. The baculovirus expression vector systems I: production and isolation of recombinant virus, p. 204-210. In J. Celi (ed.), Cell Biology Handbook 2nd Edn. Academic Press.


    King, L.A. and Possee, R.D. 1992. The baculovirus expression vector system: a laboratory guide. Chapman and Hall, London.


    King, L.A. and Possee, R.D. 1999. A novel Baculovirus Expression System. A one-step procedure for producing recombinant baculovirus expression vectors. Granted/in final processes: UK, Europe, Australia, Israel, USA, Canada.