The INDEPTH Academy is a training resource developed by the COST Action 'Impact of Nuclear Domains On Gene Expression and Plant Traits (INDEPTH)'
The INDEPTH Academy will deliver multimedia resources to inform how the spatial organisation of nuclear DNA is critical in the adaptation to environmental stresses.
Many of these resources can be found on the INDEPTH Academy YouTube Channel.
INDEPTH Academy outputs include:
- Short webinar teaching resources that introduce functional domains of the nucleus (such as the nucleolus of nuclear envelope) and explore how chromatin dynamics influence gene expression
- Masterclass tutorials in which technical experts will lead discussion-led sessions on experimental techniques that are important for study of the plant nucleus
- Standardised Protocols that are linked to INDEPTH member research activities
- Expert talks from the 2021 SEB Antwerp Main Meeting Special Session on 'Dynamic organization of the nucleus across kingdoms''.
Celia Baroux on the 'Spatial analysis of nuclear signal distribution using Imaris and DataViz'
Dimiter Prodanov and Sumit Vohra introduce their Active Segmentation tool for plant image analysis.
Ruben Gutzat provides a technical webinar on Fluorescence-Activated Nuclei Sorting (FANS).
Christophe Tatout provides a detailed description of NucleusJ 2.0 with Q+A
Stefanie Rosa provides a detailed description of smFISH with extended Q+A
Professor Hank Bass from Florida State University is hosting the INDEPTH Image Repository through the OMERO software. https://doi.org/10.24384/cqrx-5f97
Gianluca Teano discusses his research on the role of Histone 1 in chromatin organisation, which has benefited from his participation in two STSMs.
Protocol for Hi-C Chromosome Confirmation Capture. This method is adapted from research in the labs of Chang Liu and Silin Zhong. https://doi.org/10.24384/39fw-jm66
Protocol for Single Molecular Fluorescent In Situ Hybridisation. This method is adapted from research in Stefanie Rosa's lab and provides instructions for this powerful technique for analysis of gene expression https://doi.org/10.24384/4h9v-ar37
Protocol for Localising Total mRNA in Plant Cells . This method is adapted by Geraint Parry for a selection of published methodologies https://doi.org/10.24384/m5dm-he87
Protocol for Fluorescence Activated Nuclear Sorting (FANS) for RNA extraction, DNA extraction or Chromatin Immunoprecipitation. This method is adapted from protocols developed in Ortrun Mittelsten Scheid's lab https://doi.org/10.24384/fvmf-km57
Protocol for Assay for Transposase-Accessible Chromatin (ATAC-seq ). This method is adapted from a variety of protocols from the labs of Roger Deal and Kenneth Berendzen
Protocol for Membrane Yeast Two-Hybrid (MYTH) for analysis of targeted interactions or identification of novel interactors. This method is adapted from a variety of protocols, in particular from the lab of Christophe Tatout.
If you are interested in joining Action please contact the INDEPTH Chairman stating why the research program of your lab would enhance the overall activity of the Action.