INDEPTH Proteomics Training School: July 2019

Friday, 10 May 2019

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We announce the organization of the INDEPTH Training School on Plant Nuclear Proteomics and cordially invite you to participate.

The training school will take place in the laboratories of the Centre of the region Haná for Biotechnological and Agricultural Research, which are situated either in the Institute of Experimental Botany (IEB), AS CR, or Faculty of Science, Palacký University (UPOL), both on Slechtitelu street, Olomouc, Czech Republic, during July 8-12, 2019. The venue is accessible by public bus transport from the city center and main railway station.

Deadline for applications is June 15th.

The INDEPTH COST Action will support the transport and accommodation for 16 participants who work in an affiliated COST country.

This is a superb opportunity for people to learn about these cutting-edge technologies.

Download PDF with meeting details.

Objectives:

Since its introduction in the 1990s, mass spectrometry-based proteomics has evolved to a reliable tool, which helps to answer many biological questions related to the function of proteins. Similarly, flow cytometry has become very useful for the isolation of plant cell nuclei. Hence the recently developed combination of these techniques allows efficient investigations of plant nuclear proteins. The school is designed for doctoral students and researchers who need to understand and employ the techniques of isolating plant nuclei, removing DNA, nuclear protein recovery plus digestion and peptide-based protein identification using tandem mass spectrometry (MS/MS).

The program will include the following topics covered by lectures and laboratory experiments:

1) Plant sample preparation and flow cytometry of cell nuclei

2) Digestion of the nuclei by a nuclease
3) Protein recovery and proteolytic digestion
4) Peptide desalting

5) Reversed-phase liquid chromatography of peptides
6) Tandem mass spectrometry (ESI-MS/MS and MALDI-MS/MS) 7) MS/MS data processing & bioinformatics

 

Register for the workshop here: https://api.dsi.uca.fr/paybag/registration/form/event/TRAINING-SCHOOL-OLOMOUC-2019