A major bottleneck towards a better understanding of nuclear organization and function in plants is the lack of dedicated imaging and analysis procedures that meet the challenges of plant cells. WG1 aims to coordinate efforts to improve sample preparation, image acquisition and analysis approaches that meet the specific challenges of plants and will allow imaging plant nuclei in their tissue context as part of a living organism.
Workgroup 1 has established a free-to-access Image Repository hosted by OMERO at Florida State University through a collaboration with Professor Hank Bass.
Bilalovic, O, Avdagic, Z.; Omanovic, S, Besic, I, Letic, V, Tatout, C (2020) Mathematical Modelling of Ground Truth Image for 3D Microscopic Objects Using Cascade of Convolutional Neural Networks Optimized with Parameters' Combinations Generators. Symmetry, 12, 416 https://www.mdpi.com/2073-8994/12/3/416
Biel AM, Moser M, Meier I (2019) A role for plant KASH proteins in regulating
stomatal dynamics. Plant Physiol. doi:
Le Goff S, Nur Keçeli B, Jerabkova H, Heckmann S, Rutten T, Cotterell S, Schubert V, Roitinger E, Mechtler K, Franklin FCH, Tatout C, Houben A, Geelen D, Probst AV, Lermontova I (2019) The H3 histone chaperone NASPSIM3 escorts CenH3 in Arabidopsis. Plant J doi: 10.1111/tpj.14518
Dumur T, Duncan S, Graumann K, Desset S, Randall RS, Scheid OM, Prodanov D, Tatout C, Baroux C (2019) Probing the 3D architecture of the plant nucleus with microscopy approaches: challenges and solutions. Nucleus. 181-212. doi: 10.1080/19491034.2019.1644592
Rutowicz K, Lirski M, Mermaz B, Teano G, Schubert J, Mestiri I, Kroteń MA, Fabrice TN, Fritz S, Grob S, Ringli C, Cherkezyan L, Barneche F, Jerzmanowski A, Baroux C (2019) Linker histones are fine-scale chromatin architects modulating developmental decisions in Arabidopsis. Genome Biol. 20(1):157. doi: 10.1186/s13059-019-1767-3
Goto C, Hashizume S, Fukao Y, Hara-Nishimura I, Tamura K (2019) Comprehensive nuclear proteome of Arabidopsis obtained by sequential extraction. Nucleus 81-92. doi: 10.1080/19491034.2019.1603093
Monica Pradillo, David Evans and Katja Graumann (2019) The nuclear envelope in higher plant mitosis and meiosis. Nucleus DOI: 10.1080/19491034.2019.1587277
Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW (2019) Identification and characterization of genes encoding the nuclear envelope LINCcomplex in the monocot species Zea mays. J Cell Sci pii: jcs.221390. doi: 10.1242/jcs.221390
Newman-Griffis AH, Del Cerro P, Charpentier M, Meier I (2018) Medicago LINC complexes function in nuclear morphology, nuclear movement, and root nodule symbiosis. Plant Physiol. doi: 10.1104/pp.18.01111
Poulet A, Zhou X, Tamura K, Meier I, Tatout C, Graumann K, Evans, DE (2018) Computational Methods for Studying the Plant Nucleus in Gregg G Gundersen and Howard J Worman (ed.), The LINC Complex (Methods in Molecular Biology, Vol. 1840). ISBN: 9781493986903 eISBN: 9781493986910
Groves NR, Biel AM, Newman-Griffis AH, Meier I (2018) Dynamic Changes in Plant Nuclear Organization in Response to Environmental and Developmental Signals. Plant Physiol. 176(1):230-241. doi: 10.1104/pp.17.00788.
Voisin M, Vanrobays E, Tatout C (2018) Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System. Methods Mol Biol. 1840:221-235. doi: 10.1007/978-1-4939-8691-0_16
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If you are interested in joining Action please contact the INDEPTH Chairman stating why the research program of your lab would enhance the overall activity of the Action.