Professor Linda King
BSc, DPhil, FRSB
Pro Vice-Chancellor - Research and Global Partnerships
School of Biological and Medical Sciences

Role
Professor Linda King is Pro Vice-Chancellor for Research and Global Partnerships.
Linda joined Oxford Brookes in 1986 as a Lecturer in Virology and was promoted to Professor in 1998. She was Dean of the School of Life Sciences for eight years until she became Associate Dean for Research and Knowledge Exchange for the Faculty of Health and Life Sciences.
Linda completed her doctorate in molecular virology at Oxford University in 1985. Her research has focused on understanding the biology of insect viruses and on their exploitation in agriculture, medicine and biotechnology.
She is co-founding director of a joint Brookes and Natural Environment Research Council spin-out biotech company.
Having published more than 100 papers in peer-reviewed journals, reviews and book chapters, Linda has also spoken at numerous national and international meetings, including The American Society for Virology, The Society for Invertebrate Pathology, Peptalk, Global Protein Summit and Baculovirus Technology.
Research
Following the completion of my doctorate in molecular virology at Oxford University in 1985, my research has focused on various aspects of insect virology with particular emphasis on baculovirus expression systems. I have published more than 90 papers in peer-reviewed journals, reviews and book chapters, and until recently was Insect Virus Editor for The Journal of General Virology. I have spoken at numerous national and international meetings, including The American Society for Virology, The Society for Invertebrate Pathology, Peptalk, Global Protein Summit and Baculovirus Technology.
I also played an integral role in the establishment of Oxford Expression Technologies (OET), trading under Oxford Brookes Enterprises Ltd, in 1997 and continue to work as Director and Chair of SAB.
The biology and replication of insect baculoviruses in cultured insect cells and in larvae, with a focus on the role of non-essential genes encoding proteins such as P10. chitinase and cathepsin, and the trafficking of virus proteins and particles through insect cells using a range of bioimaging techniques. We are also interested in the exploitation of baculoviruses as gene expression vectors that has led to patenting of a new platform technology ‘flashBAC’ for the production of recombinant viruses and the formation of spin-off company Oxford Expression Technologies Ltd.
Groups
Projects as Principal Investigator, or Lead Academic if project is led by another Institution
- Protein nanoparticle vaccine platform for rapid response against zoonotic viruses in poultry and swine (NanoZoo) led by Waginengen University (01/10/2023 - 30/09/2026), funded by: Department of Environment, Food and Rural Affairs (DEFRA), funding amount received by Brookes: £420,557
- Effect of recombinant protein expression on baculovirus budded virus structure and infectivity (01/04/2023 - 31/03/2024), funded by: Biotechnology & Biological Sciences Research Council (BBSRC), funding amount received by Brookes: £9,326
Publications
Journal articles
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Dowall SD, Graves LP, Kennedy E, Graham VA, Alakeely RA, Chambers AP, Possee RD, King LA, Hewson R, Development of humanised antibodies for Crimean-Congo Haemorrhagic Fever virus: comparison of hybridoma-based versus phage library techniques
Journal of Immunological Methods 512 (2023) ISSN: 0022-1759 eISSN: 1872-7905 Development of humanised antibodies for Crimean-Congo Haemorrhagic Fever virus: comparison of hybridoma-based versus phage library techniques Open Access version on RADAR -
Bannach C, Ruiz Buck D, Bobby G, Graves LP, Sainan L, Chambers AC, Gan E, Arinto-Garcia R, Possee RD, King LA, Optimising recombinant baculovirus vector design for protein production in insect cells
Processes 9 (12) (2021) ISSN: 2227-9717 eISSN: 2227-9717 -
Hinsberger A, Graillot B, Blachère-Lopez C, Juliant C, Cerutti M, King LA, Possee RD, Gallardo F, Lopez-Ferber M, Tracing baculovirus AcMNPV infection using a real time method based on ANCHORTM DNA labeling technology
Viruses 12 (1) (2020) ISSN: 1999-4915 eISSN: 1999-4915 Tracing baculovirus AcMNPV infection using a real time method based on ANCHORTM DNA labeling technology Open Access version on RADAR -
Graves LP, Hughes LC, Irons SL, Possee RD, King LA, In cultured cells the baculovirus P10 protein forms two independent intracellular structures that play separate roles in occlusion body maturation and their release by nuclear disintegration
PLoS Pathogens 15 (6) (2019) ISSN: 1553-7366 eISSN: 1553-7374 In cultured cells the baculovirus P10 protein forms two independent intracellular structures that play separate roles in occlusion body maturation and their release by nuclear disintegration Open Access version on RADAR -
Graves LP, Aksular M, Alakeely RA, Ruiz Buck D, Chambers AC, Murguia-Meca F, Plata-Muñoz J-J, Hughes S, Johnson PRV, Possee RD, King LA, Improved Baculovirus Vectors for Transduction and Gene Expression in Human Pancreatic Islet Cells
Viruses 10 (10) (2018) ISSN: 1999-4915 eISSN: 1999-4915 Improved Baculovirus Vectors for Transduction and Gene Expression in Human Pancreatic Islet Cells Open Access version on RADAR -
Aksular M, Calvo-Pinilla E, Marín-López A, Ortego J, Chambers AC, King LA, Castillo-Olivares, J, A single dose of African horse sickness virus (AHSV) VP2 based vaccines provides complete clinical protection in a mouse model
Vaccine 36 (46) (2018) pp.7003-7010 ISSN: 0264-410X A single dose of African horse sickness virus (AHSV) VP2 based vaccines provides complete clinical protection in a mouse model Open Access version on RADAR -
Raza F, McGouran JF, Kessler BM, Possee RD, King LA, Phosphorylation induces structural changes in the Autographa californica nucleopolyhedrovirus P10 protein
Journal of Virology 91 (2017) ISSN: 0022-538X eISSN: 1098-5514 Phosphorylation induces structural changes in the Autographa californica nucleopolyhedrovirus P10 protein Open Access version on RADAR -
Hitchman E, Hitchman R, King LA, BacMam delivery of a protective gene to reduce renal ischemia-reperfusion injury
Human Gene Therapy 28 (9) (2017) pp.747-756 ISSN: 1043-0342 BacMam delivery of a protective gene to reduce renal ischemia-reperfusion injury Open Access version on RADAR -
Wang H, Xie J, Shreeve TG, Ma J, Pallet DW, King LA, Possee RD, Sequence Recombination and Conservation of Varroa destructor Virus-1 and Deformed Wing Virus in Field Collected Honey Bees (Apis mellifera)
PLoS ONE 8 (9) (2013) ISSN: 1932-6203 eISSN: 1932-6203 -
Locanto E, Hitchman R, King L, BacMam vector-mediated gene delivery for ischaemia reperfusion-injury
Human Gene Therapy 23 (5) (2012) pp.16-16 ISSN: 1043-0342 -
Danquah JO, Botchway S, Jeshtadi A, King LA, Direct interaction of Baculovirus Capsid Proteins VP39 and EXON0 with Kinesin-1 in insect cells determined by fluorescence resonance energy Transfer-Fluorescence Lifetime Imaging Microscopy
Journal of Virology 86 (2) (2012) pp.844-853 ISSN: 0022-538X eISSN: 1098-5514 -
Hitchman R, Possee R, King L, High-Throughput Baculovirus Expression in Insect Cells
Methods in Molecular Biology 824 (3) (2012) pp.609-627 ISSN: 1064-3745 -
Locanto E, Hitchman RB, King LA, BacMam; a novel gene delivery vector for ischaemia
Transplant International 25 (s1) (2012) pp.31-31 ISSN: 0934-0874 eISSN: 1432-2277 -
Murguia-Meca F, Plata-Munoz JJ, Hitchman RB, Danquah JO, Hughes D, Friend PJ, Fuggle SV, King LA, Baculovirus as delivery system for gene transfer during hypothermic organ preservation
Transplant International 24 (8) (2011) pp.820-828 ISSN: 0934-0874 eISSN: 1432-2277 -
Hitchman RB, Murguia-Meca F, Locanto E, Danquah J, King LA, Baculovirus as vectors for human cells and applications in organ transplantation
Journal of Invertebrate Pathology 107 (2011) pp.S49-S58 ISSN: 0022-2011 -
Locanto E, Hitchman R, King LA, Baculovirus gene delivery: a model to improve renal ischaemia
Human Gene Therapy 22 (10) (2011) pp.104-104 ISSN: 1043-0342 -
Hitchman RB, Locanto E, Possee RD, King LA, Optimizing the baculovirus expression vector system
Methods 55 (1) (2011) pp.52-57 ISSN: 1046-2023 -
Birks SM, Danquah JO, King LA, Vlasak R, Gorecki DC, Pilkington GJ, Targeting the GD3 acetylation pathway selectively induces apoptosis in glioblastoma
Neuro-Oncology 13 (9) (2011) pp.950-960 ISSN: 1522-8517 -
van Oers M, King L, The application of baculoviruses in human and veterinary medicine: An overview
Journal of Invertebrate Pathology 107 (2011) pp.1-2 ISSN: 0022-2011 -
Hitchman RB, Possee RD, Crombie AT, Chambers A, Ho K, Siaterli E, Lissina O, Sternard H, Novy R, Loomis K, Bird LE, Owens RJ, King LA, Genetic modification of a baculovirus vector for increased expression in insect cells
Cell Biology and Toxicology 26 (1) (2010) pp.57-68 ISSN: 0742-2091 -
Hitchman RB, Possee RD, Siaterli E, Richards KS, Clayton AJ, Bird LE, Owens RJ, Carpentier DCJ, King FL, Danquah JO, Spink KG, King LA, Improved expression of secreted and membrane-targeted proteins in insect cells
Biotechnology and Applied Biochemistry 56 (3) (2010) pp.85-93 ISSN: 0885-4513 -
Jeshtadi A, Burgos P, Stubbs C, Parker A, King LA, Skinner MA, Botchway S, Interaction of Poxvirus Intracellular Mature Virion Proteins with the TPR Domain of Kinesin Light Chain in Live Infected Cells Revealed by Two-Photon-Induced Fluorescence Resonance Energy Transfer Fluorescence Lifetime Imaging Microscopy
Journal of Virology 84 (24) (2010) pp.12886-12894 ISSN: 0022-538X eISSN: 1098-5514 -
Hitchman RB, Possee RD, King LA, Baculovirus expression systems for recombinant protein production in insect cells
Recent Patents on Biotechnology 3 (1) (2009) pp.46-54 ISSN: 1872-2083 eISSN: 2212-4012 -
Haines FJ, Hawes CR, Possee RD, King LA., Budded Virus Formation In Baculovirus-Infected Cells Involves The Localisation Of Gp64 Within Cholesterol-Enriched Budding Domains.
Virologica Sinica 24 (2009) pp.333-349 ISSN: 1674-0769 eISSN: 1995-820X -
Carpentier DCJ, King LA, The long road to understanding the baculovirus P10 protein
Virologica Sinica 24 (2009) pp.227-242 ISSN: 1674-0769 eISSN: 1995-820X -
Murguia-Meca F, Hitchman RB, Plata-Munoz JJ, Friend PJ, King LA, A novel baculovirus delivery tool for gene therapy in in vitro and ex vivo models of renal ischemic injury
American Journal of Transplantation 8 (2008) pp.633-633 ISSN: 1600-6135 eISSN: 1600-6143 -
Kelly BJ, Chapple SDJ, Allen C, Pritchard C, King LA, Possee RD, Extended budded virus formation and induction of apoptosis by an AcMNPV FP-25/p35 double mutant in Trichoplusia ni cells
Virus Research 133 (2008) pp.157-166 ISSN: 0168-1702 eISSN: 1872-7492 -
Possee R, Hitchman R, Richards K, Mann S, Siaterli E, Nixon C, Irving H, Assenberg R, Alderton D, Owens R J, King LA, Generation of baculovirus vectors for the high-throughput production of proteins in insect cells
Proceedings of the Phytochemical Society of Europe 101 (6) (2008) pp.1115-1122 ISSN: 978-94-010-6021-9 -
Birks S, Danquah J, Hitchman R, King L, Vlasak R, Gorecki D, Pilkington G, Manipulation of Glioma Cell Surface Gangliosides: Potential Theraputic Implications
Anticancer Research 28 (5C) (2008) pp.3214-3214 ISSN: 0250-7005 eISSN: 1791-7530 -
Carpentier DCJ, Griffiths CM, King LA, The baculovirus P10 protein of Autographa californica qucleopolyhedrovirus forms two distinct cytoskeletal-like structures and associates with polyhedral occlusion bodies during infection.
Virology 371 (2) (2008) pp.278-291 ISSN: 0042-6822 eISSN: 1178-122X -
Hitchman R, Hodgson D, King LA, Cory JS, Hails R, Possee RD, Host mediated selection of pathogen genotypes as a mechanism for the maintenance of baculovirus diversity in the field.
Journal of Invertebrate Pathology 94 (2007) pp.153-162 ISSN: 0022-2011 -
Kelly BJ, King LA, Possee RD, Chapple SDJ, Dual mutations in the Autographa californica nucleopolyhedrovirus FP-25 and p35 genes result in plasma-membrane blebbing in Trichoplusia ni cells
Journal of General Virology 87 (2006) pp.531-536 ISSN: 0022-1317 eISSN: 1465-2099 -
Burden J, Possee RD, Sait S, King LA, Hails RS, Phenotypic and genotypic characterisation of persistent baculovirus infections in populations of the cabbage moth (Mamestra brassicae) within the British Isles
Archives of Virology 151 (2006) pp.635-649 ISSN: 0304-8608 eISSN: 1432-8798 -
Saville GP, Patmanidi AL, Possee RD, King LA, Deletion of the Autographa californica nucleopolyhedrovirus chitinase KDEL motif and in vitro and in vivo analysis of the modified virus
Journal of General Virology 85 (2004) pp.821-831 ISSN: 0022-1317 eISSN: 1465-2099 -
Burden J, Nixon C, Hodgekinson A, Possee RD, Sait SM, King LA, Hails R, Covert infections as a mechanism for the long-term persistence of baculoviruses.
Ecology Letters 6 (2003) pp.524-531 ISSN: 1461-023X eISSN: 0906-7590, ESSN: 1461-0248 -
Patmanidi AL, Possee RD, King LA, Formation of P10 tubular structures during AcMNPV infection depends on the integrity of host-cell microtubules.
Virology 317 (2003) pp.308-320 ISSN: 0042-6822 eISSN: 1178-122X -
Saville GP, Thomas CJ, Possee RD, King LA, Partial redistribution of the Autographa californica nucleopolyhedrovirus chitinase in virus-infected cells accompanies mutation of the carboxy-terminal KDEL ER-retention motif
Journal of General Virology 83 (2002) pp.685-694 ISSN: 0022-1317 eISSN: 1465-2099 -
Grasela JJ, McIntosh AH, Goodman CL, Wilson LE, King LA, Expression of the green fluorescent protein carried by Autographa Californica multiple nucleopolyhedrovirus in insect cell lines
In Vitro Cellular and Developmental Biology - Animal 36 (2000) pp.205-210 ISSN: 1071-2690 eISSN: 1543-706X -
Thomas CJ, Gooday GW, King LA, Possee RD, Mutagenesis of the active site coding region of the Autographa californica nucleopolyhedrovirus chiA gene
Journal of General Virology 81 (2000) pp.1403-1411 ISSN: 0022-1317 eISSN: 1465-2099 -
Shi J, Thomas CJ, King LA, Hawes CR, Possee RD, Edwards ML, Pallett D, Cooper JI, The expression of a baculovirus-derived chitinase gene increased resistance of tobacco cultivars to brown spot (Alternaria alternata)
Annals of Applied Biology 136 (2000) pp.1-8 ISSN: 0003-4746 -
King LA, Thomas CJ, Wilkinson N, Possee RD, Expression of green fluorescent protein using baculovirus vectors
Methods in Enzymology 303 (1999) pp.394-408 ISSN: 0076-6879 eISSN: 1557-7988 -
Possee RD, Thomas CJ, King LA, The use of baculovirus vectors for the production of membrane proteins in insect cells
Biochemical Society Transactions 27 (1999) pp.928-932 ISSN: 0300-5127 eISSN: 1470-8752 -
Griffiths CM, Barnett AL, Ayres MD, Windass J, King LA, Possee RD, In vitro host range of Autographa californica nucleopolyhedrovirus recombinants lacking functional p35, iap1 or iap2
Journal of General Virology 80 (1999) pp.1055-1066 ISSN: 0022-1317 eISSN: 1465-2099 -
Thomas CJ, Brown HL, Hawes CR, Lee BY, Min MK, King LA, Possee RD, Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum
Journal of Virology 72 (1998) pp.10207-10212 ISSN: 0022-538X -
Possee RD, Barnett AL, Hawtin RE, King LA, Engineered baculoviruses for pest control
Pest Management Science 51 (1997) pp.462-470 ISSN: 1526-498X -
Hughes DS, Possee RD, King LA, Evidence for the presence of a low-level, persistent baculovirus infection of Mamestra brassicae insects
Journal of General Virology 78 (1997) pp.1801-1805 ISSN: 0022-1317 eISSN: 1465-2099 -
Wilson LE, Wilkinson N, Marlow SA, Possee RD, King LA, Identification of recombinant baculoviruses using green fluorescent protein as a selectable marker
BioTechniques 22 (1997) pp.674-681 ISSN: 0736-6205 eISSN: 1940-9818 -
King L, Appleton J V, Intuition: A critical review of the research and rhetoric
Journal of Advanced Nursing 26 (1) (1997) pp.194-202 ISSN: 0309-2402 -
Hawtin RE, Zarkowska T, Arnold K, Thomas CJ, Gooday GW, King LA, Kuzio JA, Possee RD, Liquefaction of Autographa californica nucleopolyhedrovirus-infected insects is dependent on the integrity of virus-encoded chitinase and cathepsin genes
Virology 238 (1997) pp.243-253 ISSN: 0042-6822 eISSN: 1178-122X -
Obosi LA, Hen R, Beadle DJ, Bermudez I, King LA, Mutational analysis of the mouse 5-HT7 receptor: importance of the third intracellular loop for receptor-G-protein interaction
FEBS Letters 412 (1997) pp.312-324 ISSN: 0014-5793 eISSN: 1873-3468 -
McCarroll L, King LA, Stable insect cell cultures for recombinant protein production
Current Opinion in Biotechnology 8 (1997) pp.590-594 ISSN: 0958-1669 -
Aspinwall LS, Bermudez I, King LA, Wafford KA, The interactions of hexachlorocyclohexane isomers with human gamma- aminobutyric acid(A) receptors expressed in Xenopus oocytes
The Journal of Pharmacology and Experimental Therapeutics 282 (1997) pp.1557-1564 ISSN: 0022-3565 -
Merrington CL, Kitts PA, King LA, Possee RD, An Autographa californica nucleopolyhedrovirus lef-2 mutant: Consequences for DNA replication and very late gene expression
Virology 217 (1996) pp.338-348 ISSN: 0042-6822 eISSN: 1178-122X -
Atkinson AE, Henderson J, Hawes CR, King LA, Efficient Membrane Targeting of the Chick Nicotinic Acetylcholine Receptor Alpha-subunit in Stable Insect Cell Lines
Cytotechnology 19 (1996) pp.37-42 ISSN: 0920-9069 eISSN: 1573-0778 -
Obosi LA, Schuette DG, Europe-Finner GN, Beadle DJ, Hen R, King LA, Bermudez I, Functional characterization of the Drosophila 5-HTdro1 and 5-HTdro2b serotonin receptors in insect cells - activation of a G(Alpha) s-like protein by 5-HTdro1 but lack of coupling to inhibitory G-proteins by 5-HTdro2b.
FEBS Letters 381 (4) (1996) pp.233-236 ISSN: 0014-5793 eISSN: 1873-3468 -
Henderson J, Napier RM, Lazarus CM, King LA, Transgene Expression in Insect Cells: Comparison of Transformation and Baculovirus Infection.
Molecular Biology of the Cell 7 (1996) pp.448-448 ISSN: 1939-4586 eISSN: 1939-4586 -
Henderson J, Atkinson AE, Lazarus CM, Hawes CR, Napier RM, MacDonald H, King LA, Stable Expression of Maize Auxin-binding Protein in Insect-cell Lines
FEBS Letters 371 (3) (1995) pp.293-296 ISSN: 0014-5793 eISSN: 1873-3468 -
Marlow SA, Wilson LE, Lawrie AM, Wilkinson N, King LA, Assembly of Amsacta moorei entomopoxvirus spheroidin into spheroids following synthesis in insect cells using a baculovirus vector
Journal of General Virology 79 (1988) pp.623-628 ISSN: 0022-1317 eISSN: 1465-2099
Books
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Watkins MJ, Kiyatkin N, Hughes D, Beadle DJ, King LA,
Study on the biological properties of a novel recombinant baculovirus,
British Crop Protection Council (1997) ISBN: 1901396002, 978190139600 -
King LA, Possee RD,
The Baculovirus Expression Vector System: A Laboratory Guide
,
Springer (1992) ISBN: 978-94-010-5047-0
Book chapters
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Possee RD, Chambers AC, Graves LP, Aksular M, King LA, Recent developments in the use of baculovirus expression vectors
in Bonning, B (ed.), Insect Molecular Virology: Advances and Emerging Trends, Caister Academic Press (2019) ISBN: 9781912530083 eISBN: 9781912530090 -
Chambers A, Aksular M, Graves L, Irons SL, Possee RD, King LA, Overview of the baculovirus expression system
in Current Protocols in Protein Science, Wiley (2017) Overview of the baculovirus expression system Open Access version on RADAR -
Possee R, Griffiths C, Hitchman R, Chambers A, Murguia-Meca F, Danquah J, Jeshtadi A, King L, Baculoviruses: biology, replication and exploitation
in Insect Virology, Caister Academic Press (2010) ISBN: 9781904455714 -
Haines FJ, Possee RD, King LA, Baculovirus Expression Vectors
in Encyclopedia of Virology, Elsevier (2008) ISBN: 978-0-12-374410-4 -
King LA, Possee RD, Baculovirus transfer vectors
in : Methods in Molecular Biology 338: Baculovirus and Insect Cell Expression Protocols, Humana Press (2007) ISBN: 978-1-58829-537-8 -
Hitchman RB, Possee RD, King LA, Improved Baculovirus Expression Vectors
in Expression Systems. Methods Express, Scion Publishing (2007) ISBN: 9781904842453 eISBN: 9781907904400 -
Kelly BJ, King LA, Possee RD, Introduction to Baculovirus Molecular Biology
in Baculovirus and Insect Cell Expression Protocols, Humana Press (2007) ISSN: 1064-3745 ISBN: 978-1-58829-537-8 -
Hitchman R, Possee RD, King LA, Recombinant baculovirus isolation
in Murhammer DW (ed.), Baculovirus and Insect Cell Expression Protocols, Humana Press (2007) ISBN: 978-1-58829-537-8 -
Merrington CL, King LA, Possee RD, Baculovirus Expression Systems
in Protein Expression, A Practical Approach, Oxford University Press (1999) ISBN: 0199636230, 978019963623 -
King LA, Wilkinson N, Miller G, Marlow S, Entomopoxviruses
in Miller LK, Ball LA (ed.), The Insect Viruses, Springer US (1998) ISBN: 978-1-4613-7437-4 -
King LA, Mann SG, Lawrie AM, Possee RD, The Baculovirus Expression Vector System I: Production and Isolation of Recombinant Viruses
in Celis JE (ed.), Cell Biology Handbook, Academic Press (1998) ISBN: 9780121647339 -
King LA, Marlow SA, Obosi LA, Possee RD, The Baculovirus Expression Vector systems II: Amplification and Characterization of Recombinant Viruses
in Celis JE (ed.), Cell Biology: A Laboratory Handbook, Academic press Inc (1998) ISBN: 978-0121647254
Other publications
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King LA, Baculovirus Biotechnology,
(2006) -
King LA, Possee R, A future for baculovirus insecticides? Focus on Biopesticides Plus,
(1997)
Professional information
Memberships of professional bodies
- Society for General Microbiology
- Society for Invertebrate Pathology
- Fellow of the Society of Biology