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Oxford Brookes Bioimaging Unit (OBBU) is a core facility based in the Faculty of Health and Life Sciences. Our unit has a wide range of light and electron microscopes, as well as fully-equipped electron microscopy sample preparation labs. We also have dedicated workstations for 3D image analysis. Our expertise lies in high-resolution live cell imaging and 3D electron microscopy.
VIRTUAL Bioimaging 'WALK IN' sessions: Mondays, 12 - 1 pm. No need to book, just join the following virtual meeting from your Brookes Google account: meet.google.com/ysg-hcha-byx.
Zeiss Upright confocal microscope with temperature controlled stage for live cell imaging. Allows high resolution 3D and super-resolution imaging with Airyscan technology.
Zeiss Inverted confocal microscope with temperature and CO2 controlled stage, for live cell imaging. Allows high resolution 3D and super-resolution imaging with Airyscan technology. Also equipped with Airyscan fast for greater super-resolution frame-rate.
Zeiss Axioplan fluorescence microscope equipped for DIC imaging and fluorescence imaging with DAPI, GFP and RFP filters. Equipped with low-magnification lenses for imaging of large scale tissue. Can be fitted with monochrome or colour camera.
Zeiss upright widefield fluorescence microscope. Equipped with Orca Flsh4.0 CMOS camera for high speed and sensitivity imaging. Can perform fluorescence imaging with DAPI, GFP, YFP, DsRed and CY5 filters.
Zeiss AxioZoom V16 stereoscope equipped with a number of lenses and a motorised stage. Allows fluorescence imaging with DAPI, GFP and RFP filters. Equipped with monochrome and colour cameras.
Leica MZFIII stereomicroscope equipped with fluorescence and filters for CFP, GFP, YFP and RFP. Allows imaging of large samples such as screening of transgenic organisms. Can be fitted with monochrome or colour camera.
Zeiss Merlin compact VP with Gemini column. Field emission scanning electron microscope. Fitted with Gatan 3View system for serial block face scanning electron microscopy.
Jeol JEM-1400Flash transmission electron microscope, with a Gatan OneView 16 Megapixel camera. Reaches an accelerating voltage of up to 120kV, for normal TEM imaging and electron tomography (using SerialEM).
Hitachi S-3400N scanning electron microscope. Variable pressure mode is available.
RMC Powertomes are available for producing ultrathin sections for TEM. Cryoultramicrotomy is also possible. The MT990 is available for histological sections. RMC knifemaker is used for glass knife making and is also situated in the microtomy suite.
The HPM 010 high pressure freezer is available for rapid freezing of biological samples.
The FS-8500 system is available for freeze substitution of frozen samples.
An automated critical point dryer for easy SEM specimen preparation.
Carbon coater unit with plasma cleaning and glow discharge.
Gold sputter coating for routine SEM sample preparation.
Oxford Brookes Bioimaging Unit is open to university staff and students, as well as to external users, and we provide services and/or training, as required. We can provide assistance in designing microscopy experiments.
All projects require safety evaluation/approval by the OBBU team.
To access OBBU, please contact the relevant member of staff as directed below.
For general enquiries, or for electron microscopy enquiries, contact Dr Flávia Moreira-Leite, firstname.lastname@example.org
For light microscopy enquiries, contact Dr Joseph McKenna, email@example.com
If you already have access to OBBU, use the link below to book equipment. You will need your Calpendo login and password.
Microscope booking system
If you do not have access to OBBU, or for problems accessing equipment via Calpendo, please contact: Dr Flávia Moreira-Leite, firstname.lastname@example.org.
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