• Oxford Brookes Bioimaging Unit

  • Oxford Brookes Bioimaging Unit (OBBU) is a core facility based in the Faculty of Health and Life Sciences. Our unit has a wide range of light and electron microscopes, as well as fully-equipped electron microscopy sample preparation labs. We also have dedicated workstations for 3D image analysis. Our expertise lies in high-resolution live cell imaging and 3D electron microscopy.

    Bioimaging 'WALK IN' sessions: Mondays lunch time (12 - 1 pm), at the Bioimaging office in the Sinclair Annex. No need to book, just call the Bioimaging Office from the front door of the Annex and we'll let you in for a chat!

  • Light Microscopy

    Zeiss LSM 800 with Airyscan

    Zeiss LSM800 confocal with Airyscan

    Zeiss Upright confocal microscope with temperature controlled stage for live cell imaging. Allows high resolution 3D and super-resolution imaging with Airyscan technology.

    Zeiss LSM 880 with Airyscan

    Zeiss LSM880 confocal with Airyscan

    Zeiss Inverted confocal microscope with temperature and CO2 controlled stage, for live cell imaging. Allows high resolution 3D and super-resolution imaging with Airyscan technology. Also equipped with Airyscan fast for greater super-resolution frame-rate.

    Zeiss axioplan and others

    Zeiss Axioplan

    Zeiss Axioplan fluorescence microscope equipped for DIC imaging and fluorescence imaging with DAPI, GFP and RFP filters. Equipped with low-magnification lenses for imaging of large scale tissue. Can be fitted with monochrome or colour camera.

    Zeiss AxioImager Widefield

    Zeiss AxioImager Widefield

    Zeiss upright widefield fluorescence microscope. Equipped with Orca Flsh4.0 CMOS camera for high speed and sensitivity imaging. Can perform fluorescence imaging with DAPI, GFP, YFP, DsRed and CY5 filters.

    Zeiss AxioZoom V16

    Zeiss AxioZoom V16

    Zeiss AxioZoom V16 stereoscope equipped with a number of lenses and a motorised stage. Allows fluorescence imaging with DAPI, GFP and RFP filters. Equipped with monochrome and colour cameras.

    Leica MZFLIII stereomicroscope

    Leica MZFLIII stereomicroscope

    Leica MZFIII stereomicroscope equipped with fluorescence and filters for CFP, GFP, YFP and RFP. Allows imaging of large samples such as screening of transgenic organisms. Can be fitted with monochrome or colour camera.

  • Electron Microscopy

    Electron Microsopes

    Zeiss Merlin VP compact with Gatan 3View2XP

    Zeiss Merlin VP compact with Gatan 3View2XP

    Zeiss Merlin compact VP with Gemini column. Field emission scanning electron microscope. Fitted with Gatan 3View system for serial block face scanning electron microscopy.

    Hitachi H-7650 TEM

    Jeol JEM-1400Flash

    Jeol JEM-1400Flash transmission electron microscope, with a Gatan OneView 16 Megapixel camera. Reaches an accelerating voltage of up to 120kV, for normal TEM imaging and electron tomography (using SerialEM).

    Hitachi S-3400N

    Hitachi S-3400N

    Hitachi S-3400N scanning electron microscope. Variable pressure mode is available.

    Specimen preparation equipment

    RMC Powertome PTPC and MT990 microtome

    RMC Powertome PTPC and MT990 microtome

    RMC Powertomes are available for producing ultrathin sections for TEM. Cryoultramicrotomy is also possible. The MT990 is available for histological sections. RMC knifemaker is used for glass knife making and is also situated in the microtomy suite.

    Baltec HMP 010 high pressure freezer

    Baltec HMP 010 high pressure freezer

    The HPM 010  high pressure freezer is available for rapid freezing of biological samples.

    RMC FS-8500

    RMC FS-8500

    The FS-8500 system is available for freeze substitution of frozen samples.

    Tousimis 931.GL Critical Point Dryer

    Tousimis 931.GL Critical Point Dryer

    An automated critical point dryer for easy SEM specimen preparation.

    Agar scientific carbon coater

    Agar scientific carbon coater

    Carbon coater unit with plasma cleaning and glow discharge.

    Agar scientific sputter coater

    Agar scientific sputter coater

    Gold sputter coating for routine SEM sample preparation.